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Mr Fangqi Fu
Royal Free Hospital
UCL Medical School
London
Mr Fangqi Fu profile picture
Appointment
  • Student
  • Div of Medicine
  • Faculty of Medical Sciences
Research Summary

Currently, I'm carrying out my MRes research project under the supervision of Prof David Abraham. I'm now investigating the role of LPA on fibroblast function in vitro, by using qPCR, western blotting, and ELISA. In addition, I'm also exploring whether the circulating LPA is a useful marker for fibrosis by utilizing UPLC-MS/MS.


Previously, I worked for several labs as a research assistant. In July 2020, I joined Dr Junfang Zhang’s lab, to investigate whether exosomal microRNA (miRNA) facilitates angiogenesis in hepatocellular carcinoma (HCC), by using human umbilical vein epithelial cells (HUVECs) in CCK-8 assays and wound healing assays. Meanwhile, I also participated in another project in this lab, which was screening of anti-SARS-CoV-2 nanobodies. My main task in this project was to purify those nanobodies, by Ni affinity chromatography. 

 

Furthermore, I was also asked to join Dr David Sung-Kay Chiu’s lab in June 2019, to identify which ingredient(s) in broccoli may inhibit cancer metastasis. I carried out a project in a very beginning stage of this research, in which I explored whether broccoli peptides can suppress the cell migration, by using retinal pigment epithelial (RPE) cells and wound healing assays. 

 

In addition, I joined Cure Genetics Co Ltd in November 2018, and was under the supervision of Dr Zhuo Huang, to independently reconstruct chimeric antigen receptor (CAR) plasmids and produce CD19 CAR-T cells via Gibson Assembly, as well as viral transfection by utilizing HIV-1 lentiviruses, 293 T cells and CD8+ cytotoxic T cells, respectively. 

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