Human diets are investigated by using a dietary survey and real food samples are obtained from local chain supermarkets. 10 different antibiotics including B-lactam, fluoroquinolone, macrolide, tetracyclines, and sulfonamide, were targeted to be extracted from the meat samples such as beef, chicken, pork, fish and dairy products. The measured concentration of antibiotic residues and survey results were combined together to construct different eating habits. 

Those scenarios were simulated through MIC and MBC tests and Hollow Fibre Infection Model (HFIM) to mimic a human gut model. Then, the observed AMR development from the model was identified with genetic analysis.