Mycobacterium tuberculosis (Mtb) remains one of the biggest killers throughout times and the world’s number one killer due to a single infectious agent. Yet, very little is known about Mtb RNA biology and its role in host-pathogen interactions. Research over the last two decades has demonstrated that RNA is much more than an intermediary between genes and proteins. This multi-functional molecule has turned out to perform regulatory roles that affect all levels of gene expression in all domains of life. Bacterial RNA-based regulators (riboregulators) come in different shapes and sizes including small RNAs (sRNAs), antisense RNA, 5’ leaders and riboswitches, and they form an extensive network of co- and post-transcriptional regulators that modulate and fine-tune the protein-based regulation of gene expression mediated by e.g. transcription factors. Our aim is to obtain a deeper understanding of the basic (RNA) biology of Mtb, which in time will further the understanding of gene expression control and support the development of novel diagnostics, drugs and vaccines. By combining high-throughput methods with a range of genetic and molecular biology approaches, we aim to characterize the expression, processing, degradation and regulatory role of different Mtb riboregulators.