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Investigating ciliary trafficking in the pathogenesis and treatment of ciliopathies
Two complimentary approaches are used: i) Tandem-affinity purification (TAP) and SILAC are used to identify and characterise novel ciliary trafficking proteins, and gene knockdown in cell lines and model organisms is used to test the potential of manipulating ciliary trafficking for therapy. ii) High-throughput microscopy-based small molecule screens are undertaken to identify compounds that target ciliary trafficking, and drug hits are tested for their therapeutic potential in cell lines and model organisms.
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