In Systems Neuroscience, there are many facets to our research using in vivo systems-based approaches which rely on cross-cutting electrophysiological and molecular techniques. These have produced significant contributions to our understanding as to how neural networks impact on behaviour. Several of our groups are involved with pain research forming part of the Wellcome Trust Pain Consortium (a collaboration between UCL, KCL, Imperial and Oxford). At the single cell level, several groups use in vivo patch clamp recording and imaging to understand the link between single cell excitability, network activity and behaviour and learning.