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Publication Detail
Multiphoton Processes
  • Publication Type:
    Chapter
  • Authors:
    Bain AJ
  • Publisher:
    John Wiley & Sons
  • Publication date:
    16/01/2015
  • Place of publication:
    Hoboken, NJ, USA
  • Pagination:
    279, 320
  • Volume:
    1
  • Chapter number:
    9
  • ISBN-13:
    9781119009719
  • Medium:
    Print and online
  • Book title:
    Photonics: Scientific Foundations, Technology and Applications, Volume 1
  • Language:
    English
  • Notes:
    Bain, A. J. (2015) Multiphoton Processes, in Photonics: Scientific Foundations, Technology and Applications, Volume 1 (ed D. L. Andrews), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781119009719.ch9 Publication History Published Online: 16 JAN 2015 Published Print: 30 JAN 2015 ISBN Information Print ISBN: 9781118225530 Online ISBN: 9781119009719
Abstract
Keywords: Summary This chapter provides an introduction to the fundamental physics governing the simultaneous absorption of two or more photons by molecules. Multiphoton absorption has been at the forefront of scientific research for close to five decades, first as a spectroscopic tool providing new and complimentary information on molecular structure and dynamics to conventional (single-photon) techniques. More recently, multiphoton absorption has found widespread application at the interface between the physical and life sciences through its ability to provide inherent confocal resolution in fluorescence microscopy, allowing the investigation of complex biological samples and the study of molecular interactions in live cells. Two-photon excitation techniques find widespread application in the biological sciences, perhaps the most significant contribution two-photon excited fluorescence has made to date is in fluorescence microscopy. Two-photon microscopy has been coupled with time-resolved single-photon counting detection to enable two-photon fluorescence lifetime imaging microscopy (FLIM).
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