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Publication Detail
Assessment of ocular transduction using single-stranded and self-complementary recombinant adeno-associated virus serotype 2/8
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Article
  • Authors:
    Natkunarajah M, Trittibach P, McIntosh J, Duran Y, Barker SE, Smith AJ, Nathwani AC, Ali RR
  • Publication date:
    2008
  • Pagination:
    463, 467
  • Journal:
    Gene Therapy
  • Volume:
    15
  • Issue:
    6
  • Print ISSN:
    0969-7128
  • Keywords:
    administration & dosage, Adult, Animals, Dependovirus, DNA, Complementary, Single-Stranded, Fundus Oculi, Gene Expression, Gene Therapy, Genetic Vectors, genetics, Green Fluorescent Proteins, metabolism, methods, Mice, Microscopy, Fluorescence, Pigment Epithelium of Eye, Retina, Retinal Degeneration, Retinal Ganglion Cells, therapy, Transduction, Genetic, Transgenes
Abstract
To date adeno-associated viral (AAV) vectors are the only gene therapy vectors that have been shown to efficiently transduce photoreceptor cells and have thus become the most commonly used vector for ocular transduction. Various AAV serotypes have been evaluated in the eye, the first of which was AAV2, which is able to transduce photoreceptors, retinal pigment epithelium (RPE) and retinal ganglion cells. AAV serotypes 1 and 4, as well as AAV2 pseudotyped with these capsids, only transduce the RPE. AAV serotype 5 and AAV2/5 transduce the photoreceptors as well as RPE, but not retinal ganglion cells. Here, we assessed the capacity of the novel serotype AAV2/8 to transduce various ocular tissues of the adult murine retina by administering AAV2/8 green fluorescent protein intravitreally, subretinally and intracamerally. We also determined the kinetics and efficiency of self-complementary AAV (scAAV) vectors of serotypes 2/2, 2/5 and 2/8 and compared them with single-stranded AAV (ssAAV). We found that ssAAV2/8 transduces photoreceptors and RPE more efficiently than ssAAV2/2 and ssAAV2/5, and that scAAV2/8 had faster onset and higher transgene expression than ssAAV2/8. This improved transduction efficiency might facilitate the development of improved gene therapy protocols for inherited retinal degenerations, particularly those caused by defects in photoreceptor-specific genes
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Institute of Ophthalmology
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Research Department of Haematology
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Research Department of Haematology
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Institute of Ophthalmology
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