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Publication Detail
Anti-SSA/Ro52 autoantibodies blocking the cardiac 5-HT4 serotoninergic receptor could explain neonatal lupus congenital heart block
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Article
  • Authors:
    Eftekhari P, Salle L, Lezoualc'h F, Mialet J, Gastineau M, Briand JP, Isenberg DA, Fournie GJ, Argibay J, Fischmeister R, Muller S, Hoebeke J
  • Publication date:
    10/2000
  • Pagination:
    2782, 2790
  • Journal:
    European Journal of Immunology
  • Volume:
    30
  • Issue:
    10
  • Print ISSN:
    0014-2980
  • Keywords:
    abnormalities, ABNORMALITY, ACID, ACTIVATION, adult, Aged, amino acid, Amino Acid Motifs, Amino Acid Sequence, Animal, antibodies, Antibodies, Antinuclear, Antibody, Antibody Specificity, ANTIGEN, As, Autoantibodies, Autoantigens, Autoimmune Diseases, calcium, calcium channel, Calcium Channels, cardiac, cell, CELLS, channels, chemistry, children, CHO Cells, COMMON, complications, congenital, control, Correlation, Cricetulus, Cross Reactions, enzyme, ENZYME IMMUNOASSAY, Epitopes, etiology, Female, France, FUSION PROTEIN, genetics, Hamsters, heart, Heart Block, Immunity, Maternally-Acquired, Immunoassay, immunology, Infant, Newborn, Ion Channel Gating, Ion Transport, Lupus Erythematosus, Systemic, Male, metabolism, Middle Age, Molecular Sequence Data, Myocardium, neonatal, Patient, patients, peptide, Peptide Fragments, Peptides, Pregnancy, Pregnancy Complications, PROTEIN, Proteins, Rabbits, REACTIVITY, Receptor, receptors, Receptors, Serotonin, recombinant, Recombinant Fusion Proteins, response, Ribonucleoproteins, SEQUENCE, Serotonin, serum, Support, Non-U.S.Gov't, Transfection
  • Addresses:
    UPR 9021 CNRS "Immunochimie des Peptides et des Virus", Strasbourg, France
  • Notes:
    UI - 20518840 LA - eng RN - 0 (Antibodies, Antinuclear) RN - 0 (Autoantigens) RN - 0 (Calcium Channels) RN - 0 (Peptide Fragments) RN - 0 (Receptors, Serotonin) RN - 0 (Recombinant Fusion Proteins) RN - 0 (Ribonucleoproteins) RN - 0 (SS-A antigen) RN - 158165-40-3 (serotonin 4 receptor) PT - Journal Article DA - 20001207 IS - 0014-2980 SB - IM CY - GERMANY JC - EN5
Abstract
The 52-kDa SSA/Ro (Ro52) ribonucleoprotein is an antigenic target strongly associated with the autoimmune response in mothers whose children develop neonatal lupus and congenital heart block. When sera from patients with systemic lupus erythematosus were used as autoimmune controls in an enzyme immunoassay to screen for antibodies against the human serotoninergic 5-HT4-receptor, a high correlation was found between the presence of anti-Ro52 protein antibodies in such sera and antibodies reacting with a synthetic peptide, corresponding to the second extracellular loop of the human 5-HT4 receptor (amino acid residues 165-185). Homology scanning between the 5-HT4 peptide and the sequence of the Ro52 protein indicated two potential common epitopes located between residues 365 and 396 of the Ro52 protein. Cross- reactivity was found between the peptide derived from the 5-HT4 receptor, and a peptide corresponding to residues 365-382 of the Ro52 protein. Autoantibodies, affinity-purified on the 5-HT4 receptor peptide, specifically recognized both the Ro52 protein and the 5-HT4 receptor protein in immunoblots. The affinity-purified antibodies antagonized the serotonin-induced L-type Ca channel activation on human atrial cells. This effect could explain the electrophysiological abnormalities in neonatal lupus
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