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Publication Detail
Real-time tracking of delayed-onset cellular apoptosis induced by intracellular magnetic hyperthermia
  • Publication Type:
    Journal article
  • Authors:
    Blanco-Andujar C, Ortega D, Southern P, Nesbitt SA, Thanh NT, Pankhurst QA
  • Publication date:
    01/2016
  • Pagination:
    121, 136
  • Journal:
    Nanomedicine
  • Volume:
    11
  • Issue:
    2
  • Status:
    Published
  • Country:
    England
  • Print ISSN:
    1748-6963
  • Keywords:
    apoptosis, cell death pathways, human melanoma cells, magnetic hyperthermia, magnetic nanoparticles
  • Notes:
    © Nguyễn Thị Kim Thanh. This work is licensed under a Creative Commons Attribution 4.0 License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Abstract
AIM: To assess cell death pathways in response to magnetic hyperthermia. MATERIALS & METHODS: Human melanoma cells were loaded with citric acid-coated iron-oxide nanoparticles, and subjected to a time-varying magnetic field. Pathways were monitored in vitro in suspensions and in situ in monolayers using fluorophores to report on early-stage apoptosis and late-stage apoptosis and/or necrosis. RESULTS: Delayed-onset effects were observed, with a rate and extent proportional to the thermal-load-per-cell. At moderate loads, membranal internal-to-external lipid exchange preceded rupture and death by a few hours (the timeline varying cell-to-cell), without any measurable change in the local environment temperature. CONCLUSION: Our observations support the proposition that intracellular heating may be a viable, controllable and nonaggressive in vivo treatment for human pathological conditions.
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Dept of Physics & Astronomy
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