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Publication Detail
Quantitative in vivo optical tomography of cancer progression & vasculature development in adult zebrafish.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Article
  • Authors:
    Frankel P, Nicola Lockwood, Marie-Christine Ramel, Teresa Correia , Matthew Ellis , Rachel Patel , Sebastian Brandner, Simon Arridge , Matilda Katan ,
  • Publication date:
    28/04/2016
  • Journal:
    Oncotarget
  • Country:
    US
  • Print ISSN:
    1949-2553
  • Keywords:
    Cancer, Adult Zebrafish, Optical Projection Tomography, Hepatocellular Carcinoma, KRas
Abstract
We describe a novel approach to study tumour progression and vasculature development in vivo via global 3-D fluorescence imaging of live non-pigmented adult zebrafish utilising angularly multiplexed optical projection tomography with compressive sensing (CS-OPT). This “mesoscopic” imaging method bridges a gap between established ~µm resolution 3-D fluorescence microscopy techniques and ~mm-resolved whole body planar imaging and diffuse tomography. Implementing angular multiplexing with CS-OPT, we demonstrate the in vivo global imaging of an inducible fluorescently labelled genetic model of liver cancer in adult non-pigmented zebrafish that also present fluorescently labelled vasculature. In this disease model, addition of a chemical inducer (doxycycline) drives expression of eGFP tagged oncogenic K-RASV12 in the liver of immune competent animals. We show that our novel in vivo global imaging methodology enables non-invasive quantitative imaging of the development of tumour and vasculature throughout the progression of the disease, which we have validated against established 2 methods of pathology including immunohistochemistry. We have also demonstrated its potential for longitudinal imaging through a study of vascular development in the same zebrafish from early embryo to adulthood. We believe that this instrument, together with its associated analysis and data management tools, constitute a new platform for in vivo cancer studies and drug discovery in zebrafish disease models.
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