UCL  IRIS
Institutional Research Information Service
UCL Logo
Please report any queries concerning the funding data grouped in the sections named "Externally Awarded" or "Internally Disbursed" (shown on the profile page) to your Research Finance Administrator. Your can find your Research Finance Administrator at https://www.ucl.ac.uk/finance/research/rs-contacts.php by entering your department
Please report any queries concerning the student data shown on the profile page to:

Email: portico-services@ucl.ac.uk

Help Desk: http://www.ucl.ac.uk/ras/portico/helpdesk
Publication Detail
Efficient gene delivery to the adult and fetal CNS using pseudotyped non-integrating lentiviral vectors
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Article
  • Authors:
    Rahim AA, Wong AM, Howe SJ, Buckley SM, costa-Saltos AD, Elston KE, Ward NJ, Philpott NJ, Cooper JD, Anderson PN, Waddington SN, Thrasher AJ, Raivich G
  • Publication date:
    04/2009
  • Pagination:
    509, 520
  • Journal:
    Gene Ther.
  • Volume:
    16
  • Issue:
    4
  • Keywords:
    Adult, article, Brain, Lentivirus, Nervous System, Research, therapy, Child, health, immunology, Lentivirus
  • Addresses:
    Perinatal Brain Protection and Repair Group, Department of Obstetrics and Gynaecology, University College London, London, UK
  • Notes:
    DA - 20090409IS - 1476-5462 (Electronic)LA - engPT - Journal ArticlePT - Research Support, Non-U.S. Gov'tSB - IM
Abstract
Non-integrating lentiviral vectors show considerable promise for gene therapy applications as they persist as long-term episomes in non-dividing cells and diminish risks of insertional mutagenesis. In this study, non-integrating lentiviral vectors were evaluated for their use in the adult and fetal central nervous system of rodents. Vectors differentially pseudotyped with vesicular stomatitis virus, rabies and baculoviral envelope proteins allowed targeting of varied cell populations. Efficient gene delivery to discrete areas of the brain and spinal cord was observed following stereotactic administration. Furthermore, after direct in utero administration (E14), sustained and strong expression was observed 4 months into adulthood. Quantification of transduction and viral copy number was comparable when using non-integrating lentivirus and conventional integrating vector. These data support the use of non-integrating lentiviral vectors as an effective alternative to their integrating counterparts in gene therapy applications, and highlight their potential for treatment of inherited and acquired neurological disorders
Publication data is maintained in RPS. Visit https://rps.ucl.ac.uk
 More search options
UCL Researchers Show More
Author
Cell & Developmental Biology
Author
Maternal & Fetal Medicine
Author
Pharmacology
Author
Infection, Immunity & Inflammation Dept
Author
Maternal & Fetal Medicine
University College London - Gower Street - London - WC1E 6BT Tel:+44 (0)20 7679 2000

© UCL 1999–2011

Search by