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Publication Detail
Adapting atomic force microscopy for cell biology
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Article
  • Authors:
    Lehenkari PP, Charras GT, Nykanen A, Horton MA
  • Publication date:
    02/2000
  • Pagination:
    289, 295
  • Journal:
    Ultramicroscopy
  • Volume:
    82
  • Issue:
    1-4
  • Print ISSN:
    0304-3991
  • Keywords:
    Animal, BINDING, biology, bone, Bone cells, Calcitonin, cell, CELLS, chemistry, Force, Glass, Glasses, Head, imaging, instrumentation, interaction, interface, living, medicine, Methods, Microscopy, Microscopy, Atomic Force, osteoclasts, RANGE, rats, Receptor, receptors, Receptors, Calcitonin, SCAN, Support, Non-U.S.Gov't, SYSTEM, UK, ultrastructure
  • Addresses:
    Department of Medicine, Bone and Mineral Centre, The Rayne Institute, University College London, UK
  • Notes:
    UI - 20203725 LA - eng RN - 0 (Receptors, Calcitonin) RN - 9007-12-9 (Calcitonin) PT - Journal Article DA - 20000425 IS - 0304-3991 SB - IM CY - NETHERLANDS JC - WNA
Abstract
We present details of our AFM modifications to produce an adaptable imaging system for the cell biologist. We have designed and validated a new inverted microscope interface and a scan head with increased Z- range, based upon the TopoMetrix Explorer AFM. We have utilised these changes, together with home-made glass ball cantilevers, to obtain topographical information over cells with large Z-dimension (over 15 microm high), and mapped calcitonin-calcitonin receptor binding forces in living bone cells. We conclude that modified AFM can be used to evaluate intermolecular events in living cells and that this approach will ensure general application to the study of receptor-ligand interactions under truly physiological conditions
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