UCL  IRIS
Institutional Research Information Service
UCL Logo
Please report any queries concerning the funding data grouped in the sections named "Externally Awarded" or "Internally Disbursed" (shown on the profile page) to your Research Finance Administrator. Your can find your Research Finance Administrator at http://www.ucl.ac.uk/finance/research/post_award/post_award_contacts.php by entering your department
Please report any queries concerning the student data shown on the profile page to:

Email: portico-services@ucl.ac.uk

Help Desk: http://www.ucl.ac.uk/ras/portico/helpdesk
Publication Detail
Assessment of Cellular Redox State Using NAD(P)H Fluorescence Intensity and Lifetime.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Article
  • Authors:
    Blacker TS, Berecz T, Duchen MR, Szabadkai G
  • Publication date:
    20/01/2017
  • Journal:
    Bio-protocol
  • Volume:
    7
  • Issue:
    2
  • Medium:
    Print
  • Language:
    eng
  • Addresses:
    Consortium for Mitochondrial Research, Department of Cell & Developmental Biology, University College London, London, United Kingdom; Department of Physics & Astronomy, University College London, London, United Kingdom.
Abstract
NADH and NADPH are redox cofactors, primarily involved in catabolic and anabolic metabolic processes respectively. In addition, NADPH plays an important role in cellular antioxidant defence. In live cells and tissues, the intensity of their spectrally-identical autofluorescence, termed NAD(P)H, can be used to probe the mitochondrial redox state, while their distinct enzyme-binding characteristics can be used to separate their relative contributions to the total NAD(P)H intensity using fluorescence lifetime imaging microscopy (FLIM). These protocols allow differences in metabolism to be detected between cell types and altered physiological and pathological states.
Publication data is maintained in RPS. Visit https://rps.ucl.ac.uk
 More search options
UCL Researchers
Author
Dept of Physics & Astronomy
Author
Cell & Developmental Biology
Author
Cell & Developmental Biology
University College London - Gower Street - London - WC1E 6BT Tel:+44 (0)20 7679 2000

© UCL 1999–2011

Search by