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Publication Detail
Increased expression of lipid rafts as marker of CD4+ T cells activation correlated with disease activity score in patients with primary Sjögren’s syndrome (pSS)
  • Publication Type:
  • Authors:
    Thompson NL, Gandhi A, Radmore R, Gupta V, Cho S, Isenberg DA, Jury E, Ciurtin C
  • Presented date:
  • Presented at:
    The British Society for Rheumatology 2017
  • Location:
Introduction: It is recognised that CD4+ T helper (Th) cells play a crucial role in the pathogenesis of pSS. Previous studies identified a predominance of Th1 phenotype, in particular in pSS patients with increased histological scores and presence of serum autoantibodies. We aimed to identify the peripheral T cell phenotype abnormalities in patients with pSS compared to secondary SS associated with systemic lupus erythematosus (SS/SLE) and SLE alone, and correlate them with clinical and serological parameters, and lipid raft expression as marker of T cell activation. Our hypothesis is that disturbance in the functional properties of peripheral blood T cells can contribute to the immunopathogenesis of SS, and could have therapeutic potential. Methods: Blood samples and clinical and laboratory parameters from 32 patients with pSS, SLE and SS/SLE and 13 age/sex matched HC were obtained. Flow-cytometry was used to perform T cell immunophenotyping and lipid-raft expression analyses. Results: While there were fewer significant differences observed in the CD4+ T cells population between healthy control and the disease groups, there were several observed in the CD8+ T cells population. There was an increase in T regulatory cells (CD4+CD25+CD127-) in pSS and SLE in comparison to HC, with a significant increase (p=0.0082) in SS/SLE. A decreased in naïve (CD8+CD27-CD45RA+), activated (CD8+CD25+CD127-) and T responders cells (CD8+CD25-CD127+) (p=0.0266) were observed in SLE compared to HC. Significant increases (p=0.0196) in effector memory cells (CD8+CD27-CD45RA-) and (p=0.0196) in unknown (CD8+CD25-CD127-) were also identified. Total lipid raft expression in CD4+ T cells correlated positively with the disease activity score in pSS (ESSDAI) (p=0.0305, r =0.4617), while global BILAG score correlated positively with CD4+CD25-CD127+ T responder in SLE patients (p=0.0235, r=0.7026). Conclusion: Patients with pSS, SS/SLE and SLE have different T cell phenotype. The most significant T cell abnormalities were found in patients with SLE, however a significant correlation between lipid raft expression as marker of cell activation and disease activity score was found only in pSS patients. This is particularly relevant for therapeutic purposes, as CTLA-4 blockade is mediated by lipid rafts, and could provide in further larger study the possibility to identify which pSS patients are most likely to respond to abatacept.
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