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Publication Detail
Human monoclonal anti-phospholipid antibodies selectively bind to membrane phospholipid and beta2-glycoprotein I (beta2-GPI) on apoptotic cells
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Article
  • Authors:
    Pittoni V, Ravirajan CT, Donohoe S, MacHin SJ, Lydyard PM, Isenberg DA
  • Publication date:
    03/2000
  • Pagination:
    533, 543
  • Journal:
    Clinical and Experimental Immunology
  • Volume:
    119
  • Issue:
    3
  • Print ISSN:
    0009-9104
  • Keywords:
    ACT, AFFINITIES, affinity, analysis, Animal, antibodies, Antibodies, Antiphospholipid, Monoclonal, Antibody, Antibody Specificity, apoptosis, As, beta, BINDING, cell, Cell Membrane, CELLS, EPITOPE, Epitopes, Flow Cytometry, glycoprotein, Glycoproteins, immunology, in vivo, in-vivo, interaction, Lipids, MECHANISM, medicine, membrane, Membrane Lipids, mice, PATHOLOGICAL IMPLICATIONS, Patient, patients, Phospholipids, Rheumatology, SPECIFICITIES, SPECIFICITY, Support, Non-U.S.Gov't, Syndrome, U937 Cells, UK
  • Addresses:
    Centre for Rheumatology/Bloomsbury Rheumatology Unit, Department of Medicine and Department of Haematology, University College London, London, UK
  • Notes:
    UI - 20156200 LA - eng RN - 0 (Antibodies, Antiphospholipid) RN - 0 (Antibodies, Monoclonal) RN - 0 (Glycoproteins) RN - 0 (Membrane Lipids) RN - 0 (Phospholipids) RN - 0 (beta 2-glycoprotein I) PT - Journal Article DA - 20000424 IS - 0009-9104 SB - IM CY - ENGLAND JC - DD7
Abstract
The ability of an anti-phospholipid (LJ1) and an anti-beta2-GPI (RSP- 57) human MoAb to bind to apoptotic but not viable cells was demonstrated in this study. Both MoAbs were derived from patients with systemic lupus erythematosus and anti-phospholipid antibody syndrome. The parallel analysis of the specificity and affinity of four anti- phospholipid human MoAbs suggests that the binding of LJ1 MoAb to apoptotic cells is a specific property of this MoAb. RSP-57 MoAb recognizes apoptotic cells through beta2-GPI which becomes available for binding after the interaction with negatively charged phospholipids. This observation provides evidence that the binding of human anti-phospholipid antibodies to apoptotic cells occurs in both a beta2-GPI-dependent and independent way and involves a restricted group of epitopes. The finding that LJ1 and RSP-57 MoAbs bind apoptotic cells underlines the property of these MoAbs to act as cell membrane markers of apoptosis. Major pathological implications derive from the observation that LJ1 and RSP-57 MoAbs recognize epitopes expressed on 'early' apoptotic cells. The interference with the in vivo clearance and processing of apoptotic cells is a potential pathogenic mechanism of these antibodies
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