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Publication Detail
Stimulated Emission Depletion and Fluorescence Correlation Spectroscopy of a Branched Quadrupolar Chromophore. - art. no. 70300S
  • Publication Type:
    Conference
  • Authors:
    Armoogum DA, Marsh RJ, Nicolaou N, Mongin O, Blanchard-Desce M, Bain AJ
  • Publisher:
    SPIE-INT SOC OPTICAL ENGINEERING
  • Publication date:
    2008
  • Pagination:
    70300S-1, 70300s-10
  • Published proceedings:
    NANOPHOTONIC MATERIALS V
  • Volume:
    7030
  • Editors:
    Gaburro Z,Cabrini S,Talapin D
  • ISBN-13:
    978-0-8194-7250-2
  • Name of conference:
    Conference on Nanophotonic Materials V
  • Conference place:
    San Diego, CA
  • Conference start date:
    10/08/2008
  • Conference finish date:
    12/08/2008
  • Print ISSN:
    0277-786X
  • Language:
    EN
  • Keywords:
    Stimulated Emission Depletion, Fluorescence Correlation Spectroscopy, Single Molecule, Non-radiative relaxation, DYNAMICS, MICROSCOPY, EXCITATION, PROTEINS, STATES
Abstract
Stimulated emission depletion (STED) and single molecule fluorescence correlation spectroscopy (FCS) are used to determine stimulated emission cross-sections and investigate non-radiative relaxation in a branched quadrupolar chromophore (OM77). The results are used as inputs to simulations of single molecule STED by which the feasibility of STED control of the single molecule fluorescence cycle call be assessed. Single molecule STED in OM77 is shown to be readily achievable; however its effectiveness in reducing triplet trapping is apparently mediated by fast non-radiative relaxation processes other than intersystem crossing and rapid quenching of the triplet state in a non-deoxygenated environment.
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