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Publication Detail
Integration of Atomic Force and Confocal Microscopy
Abstract
An atomic force (AFM) and confocal microscope have been integrated by adapting commercially available systems. The set-up has been validated using green fluorescent protein (GFP)-actin transfected melanoma cells to examine the cellular topography in contact imaging mode simultaneously with fluorescence detection of the actin cytoskeleton in living cells. The F-actin distribution ascertained by both techniques showed a co-incident distribution. This experimental system should enable simultaneous measurements of fluorescence distribution with AFM-derived data.
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