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Publication Detail
Signal transducer and activator of transcription-1 and heat shock factor-1 interact and activate the transcription of the Hsp-70 and Hsp-90beta gene promoters.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    Stephanou A, Isenberg DA, Nakajima K, Latchman DS
  • Publication date:
    15/01/1999
  • Pagination:
    1723, 1728
  • Journal:
    J Biol Chem
  • Volume:
    274
  • Issue:
    3
  • Status:
    Published
  • Country:
    United States
  • Print ISSN:
    0021-9258
  • PII:
    S0021-9258(19)88290-X
  • Language:
    eng
  • Keywords:
    Binding Sites, DNA-Binding Proteins, Gene Expression Regulation, HSP70 Heat-Shock Proteins, HSP90 Heat-Shock Proteins, Heat Shock Transcription Factors, Heat-Shock Proteins, Humans, Interferon-gamma, Interleukin-6, Promoter Regions, Genetic, STAT1 Transcription Factor, STAT3 Transcription Factor, Signal Transduction, Trans-Activators, Transcription Factors, Transcription, Genetic, Transcriptional Activation, Transfection, Tumor Cells, Cultured
Abstract
We have previously demonstrated that interleukin-6 (IL-6) increases the levels of the heat shock protein 90 (Hsp-90) and activates the Hsp-90beta promoter via the IL-6-activated transcription factors NF-IL6 and signal transducer and activator of transcription-3 (STAT-3). Here, we show that interferon-gamma (IFN-gamma) treatment increases the levels of Hsp-70 and Hsp-90 and also enhances the activity of the Hsp-70 and Hsp-90beta promoters with these effects being dependent on activation of the STAT-1 transcription factor by IFN-gamma. These effects were not seen in a STAT-1-deficient cell line, indicating that IFN-gamma modulates Hsp induction via a STAT-1-dependent pathway. The effect of IFN-gamma/STAT-1 was mediated via a short region of the Hsp-70/Hsp-90 promoters, which also mediates the effects of NF-IL6 and STAT-3 and can bind STAT-1. This region also contains a binding site for the stress-activated transcription factor HSF-1. We show that STAT-1 and HSF-1 interact with one another via a protein-protein interaction and produce a strong activation of transcription, which is in contrast to our previous finding that STAT-3 and HSF-1 antagonize one another. To our knowledge this is the first report of HSF-1 interacting directly via a protein-protein interaction with another transcription factor. Such protein-protein interactions and the binding of a number of different stress and cytokine-activated transcription factors to a short region of the Hsp-90 and Hsp-70 gene promoters are likely to play a very important role in Hsp gene activation by non-stressful stimuli and the integration of these responses with the stress response of these genes.
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