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Publication Detail
Stability of blood-based biomarkers of Alzheimer's disease over multiple freeze-thaw cycles
  • Publication Type:
    Journal article
  • Publication Sub Type:
  • Authors:
    Keshavan A, Heslegrave A, Zetterberg H, Schott JM
  • Publication date:
  • Pagination:
    448, 451
  • Journal:
    Alzheimer's and Dementia: Diagnosis, Assessment and Disease Monitoring
  • Volume:
  • Status:
© 2018 The Authors Introduction: Freeze-thaw instability may contribute to preanalytical variation in blood-based biomarker studies. We investigated the effects of up to four freeze-thaw cycles on single molecule array immunoassays of serum neurofilament light chain and plasma total tau, amyloid β 1–40 (Aß40), and Aβ 1–42 (Aβ42). Methods: Individuals who had peripheral venepuncture during investigation of suspected neurodegenerative disease were recruited. After standardized preprocessing, 200 μL of plasma and serum aliquots were stored at −80°C within 60 minutes. Aliquots underwent one to four freeze-thaw cycles. Results: There was no significant difference across four freeze-thaw cycles for serum neurofilament light chain (n = 12), plasma total tau (n = 11), or plasma Aβ42(n = 12). For plasma Aβ40(n = 14), there were significant median reductions by ratios of.96 and.92 at the third and fourth cycles, respectively. Discussion: Up to four freeze-thaw cycles do not influence single molecule array blood biomarkers of neurofilament light chain, total tau, or Aβ42, with at most minor reductions in Aβ40.
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