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Publication Detail
Super-resolution fight club: A broad assessment of 2D & 3D single-molecule localization microscopy software
  • Publication Type:
    Working discussion paper
  • Authors:
    Sage D, Pham T-A, Babcock H, Lukes T, Pengo T, Chao J, Velmurugan R, Herbert A, Agrawal A, Colabrese S, Wheeler A, Archetti A, Rieger B, Ober R, Hagen G, Sibarita J-B, Ries J, Henriques R, Unser M, Holden S
  • Publication date:
    04/07/2018
  • Status:
    Published
Abstract
ABSTRACT With the widespread uptake of 2D and 3D single molecule localization microscopy, a large set of different data analysis packages have been developed to generate super-resolution images. To guide researchers on the optimal analytical software for their experiments, we have designed, in a large community effort, a competition to extensively characterise and rank these options. We generated realistic simulated datasets for popular imaging modalities – 2D, astigmatic 3D, biplane 3D, and double helix 3D – and evaluated 36 participant packages against these data. This provides the first broad assessment of 3D single molecule localization microscopy software, provides a holistic view of how the latest 2D and 3D single molecule localization software perform in realistic conditions, and ultimately provides insight into the current limits of the field.
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