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Publication Detail
Cross-reaction of lupus anti-dsDNA antibodies with protein translation factor EF-2
  • Publication Type:
    Journal article
  • Publication Sub Type:
  • Authors:
    Alberdi F, Dadone J, Ryazanov A, Isenberg DA, Ravirajan C, Reichlin M
  • Publication date:
  • Pagination:
    293, 300
  • Journal:
    Clinical Immunology
  • Volume:
  • Issue:
  • Print ISSN:
  • Keywords:
    Animal, anti-DNA, anti-DNA antibodies, antibodies, Antibodies, Antinuclear, Antibodies, Monoclonal, Antibody, Antibody Specificity, ANTIGEN, As, ASSAY, Autoantigens, Autoimmune Diseases, Cattle, cell, CELLS, Cellular, chemistry, Comparative Study, Cross Reactions, culture, DNA, drug effects, DYSFUNCTION, experiments, health, Hela Cells, Id, IM, IMMUNOGLOBULIN, IMMUNOGLOBULIN G, immunology, in vitro, In-vitro, INHIBIT, inhibition, intracellular, isolation & purification, LA, liver, Lupus, Lupus Erythematosus, Systemic, May, N-terminal, peptide, Peptide Elongation Factor 2, pharmacology, PROTEIN, protein synthesis, Proteins, Rabbits, report, ribosomal, Ribosomal Proteins, Role, S1, sequencing, SPECIFICITIES, SPECIFICITY, STATE, STATES, Support, U.S.Gov't, P.H.S., synthesis, target, Tissue, Tissue Culture, translation, Translation, Genetic, united, United States, UNITED-STATES, VITRO, western blot
  • Notes:
    UI - 21112174 LA - eng RN - 0 (Antibodies, Antinuclear) RN - 0 (Antibodies, Monoclonal) RN - 0 (Autoantigens) RN - 0 (Immunoglobulin G) RN - 0 (Peptide Elongation Factor 2) RN - 0 (Ribosomal Proteins) RN - 0 (ribosomal protein S1) RN - 9007-49-2 (DNA) PT - Journal Article ID - R01 AR43975/AR/NIAMS DA - 20010222 IS - 1521-6616 SB - IM CY - United States
This report elucidates a new cross-reactive intracellular target of anti-dsDNA antibodies. Previous experiments have demonstrated that some anti-dsDNA antibodies penetrate cells grown in tissue culture and all inhibit in vitro translation. Data implicate a cross-reactive antigen directly involved in protein synthesis: elongation factor-2 (EF-2). EF- 2 was identified by N-terminal sequencing of a band identified with an antibody to the ribosomal protein S1 from Leuconostoc lactis in Western blot assay. Anti-DNA antibodies bind directly to purified EF-2 from bovine liver in dot blot assays. Anti-dsDNA antibodies were shown to inhibit in vitro translation. This inhibiting effect of anti-dsDNA antibodies was partially restored by EF-2 and abrogated by dsDNA, suggesting this cross-reactive specificity. These data demonstrate a cross-reaction between anti-dsDNA antibodies and EF-2 which may lead to cellular dysfunction, as evidenced by inhibition of protein synthesis, and provide a direct pathogenic role for cell penetrating anti-dsDNA antibodies
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