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Publication Detail
Fluorescence lifetime imaging in turbid media
  • Publication Type:
    Chapter
  • Authors:
    Soloviev VY, Correia TM, Arridge SR
  • Publication date:
    01/01/2014
  • Pagination:
    283, 319
  • ISBN-13:
    9781439861677
  • Status:
    Published
  • Book title:
    Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics
Abstract
© 2015 by Taylor & Francis Group, LLC. Fluorescence optical tomography (fDOT) is a functional and molecular imaging modality, which consists of illuminating the object of study using a light source in the visible or near-infrared (NIR) wavelength range to excite a fluorescent probe and measuring emitted fluorescence light, and possibly excitation light, in order to reconstruct spatial maps of fluorescence parameters, such as lifetime and fluorescence yield, which is linearly related to the quantum yield and absorption coefficient of a fluorophore (Gao et al. 2010; Ntziachristos et al. 2005). Light in the NIR region is preferred because it is known to penetrate deeper into biological tissue than visible light due to the low absorption by the main tissue chromophores, such as water, oxyhemoglobin, and deoxyhemoglobin. Usually, fDOT systems with the ability to measure fluorescent lifetimes are known as fluorescence lifetime imaging microscopy (FLIM) systems.
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