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Publication Detail
The use of thin slices of myocardium for recording the currents across single ion channels
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    Burnashev NA, Edwards FA, Verkhratskiǐ AN
  • Publication date:
  • Pagination:
    119, 122
  • Journal:
    Fiziologicheskii Zhurnal
  • Volume:
  • Issue:
  • Status:
  • Print ISSN:
Thin cardiac slices (100-200 microns) from newborn (1-14 days old) rat heart ventricles were used for patch clamp recordings. High resistance seals (10-50 GOhms) between patch-clamp pipettes and the membrane of cardiac cells as well as classical patch-clamp configurations can be achieved on this preparation without any enzymatic treatment of tissue. Resisting potential for cardiac cells measured in whole-cell configuration ranged between -30 and -65 mV. Averaged sodium currents and single inward rectifying potassium elementary currents recorded in cell-attached mode displayed basic features similar to those previously reported for isolated rat ventricular cells. Application of the method described here in cardiac electrophysiology will allow patch-clamp studies on heart cells without the complicated procedures of cell isolation. In addition, the uncertainty associated with enzyme treatment can be avoided. In future, this technique could be a new tool for studying electrophysiological properties of heart cells in situ.
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