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Publication Detail
Patch-clamp recordings on rat cardiac muscle slices
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    Burnashev NA, Edwards FA, Verkhratsky AN
  • Publication date:
    01/09/1990
  • Pagination:
    123, 125
  • Journal:
    Pflügers Archiv European Journal of Physiology
  • Volume:
    417
  • Issue:
    1
  • Status:
    Published
  • Print ISSN:
    0031-6768
Abstract
Thin slices (100-200 μm) from cardiac ventricles of newborn (1-14 days old) rats were used for patch-clamp recordings. High resistance seals (>10 GOhms) could be obtained on this preparation without enzymatic treatment of tissue. Resting potential for cardiac fibres, measured in whole-cell configuration, ranged between -30 and -65 mV. Averaged sodium currents and inward rectifying potassium single-channel currents, recorded in cell-attached mode, displayed similar features to those previously reported for isolated rat ventricular cells. Application of patch-clamp techniques to cardiac slices allows single-channel recordings without complicated procedures of cell isolation. Moreover, possible alteration of channel properties caused by proteolytic enzymes can be avoided. This technique provides a new tool for studying electrophysiological properties of heart cells in situ. © 1990 Springer-Verlag.
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