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Publication Detail
High-speed volumetric imaging of neuronal activity in freely moving rodents
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Article
  • Authors:
    Skocek O, Nöbauer T, Weilguny L, Martínez Traub F, Xia CN, Molodtsov MI, Grama A, Yamagata M, Aharoni D, Cox DD, Golshani P, Vaziri A
  • Publication date:
    07/05/2018
  • Pagination:
    429, 432
  • Journal:
    Nature Methods
  • Volume:
    15
  • Issue:
    6
  • Status:
    Published
  • Print ISSN:
    1548-7091
Abstract
© 2018 The Author(s). Thus far, optical recording of neuronal activity in freely behaving animals has been limited to a thin axial range. We present a head-mounted miniaturized light-field microscope (MiniLFM) capable of capturing neuronal network activity within a volume of 700 × 600 × 360 μm3 at 16 Hz in the hippocampus of freely moving mice. We demonstrate that neurons separated by as little as ∼15 μm and at depths up to 360 μm can be discriminated.
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