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Publication Detail
Video rate volumetric Ca2+ imaging across cortex using seeded iterative demixing (SID) microscopy
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Article
  • Authors:
    Nöbauer T, Skocek O, Pernía-Andrade AJ, Weilguny L, Martínez Traub F, Molodtsov MI, Vaziri A
  • Publication date:
    26/06/2017
  • Pagination:
    811, 818
  • Journal:
    Nature Methods
  • Volume:
    14
  • Issue:
    8
  • Status:
    Published
  • Print ISSN:
    1548-7091
Abstract
© 2017 Nature America, Inc., part of Springer Nature. All rights reserved. Light-field microscopy (LFM) is a scalable approach for volumetric Ca2+ imaging with high volumetric acquisition rates (up to 100 Hz). Although the technology has enabled whole-brain Ca2+ imaging in semi-transparent specimens, tissue scattering has limited its application in the rodent brain. We introduce seeded iterative demixing (SID), a computational source-extraction technique that extends LFM to the mammalian cortex. SID can capture neuronal dynamics in vivo within a volume of 900 × 900 × 260 μm located as deep as 380 μm in the mouse cortex or hippocampus at a 30-Hz volume rate while discriminating signals from neurons as close as 20 μm apart, at a computational cost three orders of magnitude less than that of frame-by-frame image reconstruction. We expect that the simplicity and scalability of LFM, coupled with the performance of SID, will open up a range of applications including closed-loop experiments.
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