Institutional Research Information Service
UCL Logo
Please report any queries concerning the funding data grouped in the sections named "Externally Awarded" or "Internally Disbursed" (shown on the profile page) to your Research Finance Administrator. Your can find your Research Finance Administrator at https://www.ucl.ac.uk/finance/research/rs-contacts.php by entering your department
Please report any queries concerning the student data shown on the profile page to:

Email: portico-services@ucl.ac.uk

Help Desk: http://www.ucl.ac.uk/ras/portico/helpdesk
Publication Detail
Determination of ATP, ADP, and AMP Levels by Reversed-Phase High-Performance Liquid Chromatography in Cultured Cells
  • Publication Type:
  • Authors:
    Menegollo M, Tessari I, Bubacco L, Szabadkai G
  • Publisher:
    Springer Nature
  • Publication date:
  • Place of publication:
    New York (NY), USA
  • Pagination:
    223, 232
  • Volume:
  • Chapter number:
  • Series:
    Methods in Molecular Biology
  • Editors:
    Raffaello A,Vecellio Reane D
  • ISBN-13:
  • Status:
  • Book title:
    Calcium Signalling: Methods and Protocols
  • Language:
  • Keywords:
    Reversed-phase high-performance liquid chromatography (RP-HPLC), ATP/ADP ratio, Energy charge, Glycolysis, Oxidative phosphorylation, Ca²⁺ signal
Cytoplasmic and mitochondrial Ca²⁺ signals couple cellular ATP production to activity-related energy demand. In order to accurately determine the bioenergetic effect of Ca²⁺ signals, cellular energy charge, i.e., the compound ratio of the phosphorylated adenine nucleotides AMP, ADP, and ATP, should be estimated. Reversed-phase high-performance liquid chromatography (RP-HPLC) allows the rapid separation and quantitation of these molecules. Here we describe a protocol applied in our laboratories to quantify ATP, ADP, and AMP nucleotides in cellular extracts.
Publication data is maintained in RPS. Visit https://rps.ucl.ac.uk
 More search options
UCL Researchers
Cell & Developmental Biology
University College London - Gower Street - London - WC1E 6BT Tel:+44 (0)20 7679 2000

© UCL 1999–2011

Search by