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Publication Detail
Overview of quantitative LC-MS techniques for proteomics and activitomics.
  • Publication Type:
    Chapter
  • Authors:
    Timms JF, Cutillas PR
  • Publication date:
    01/2010
  • Pagination:
    19, 45
  • Volume:
    658
  • Medium:
    Print
  • Status:
    Published
  • Language:
    eng
  • Keywords:
    Animals, Humans, Proteins, Chromatography, Liquid, Staining and Labeling, Proteomics, Protein Processing, Post-Translational, Mass Spectrometry
  • Addresses:
    Cancer Proteomics Laboratory, EGA Institute for Women's Health, University College London, London, UK.
Abstract
LC-MS is a useful technique for protein and peptide quantification. In addition, as a powerful tool for systems biology research, LC-MS can also be used to quantify post-translational modifications and metabolites that reflect biochemical pathway activity. This review discusses the different analytical techniques that use LC-MS for the quantification of proteins, their modifications and activities in a multiplex manner.
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