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Publication Detail
Automating multimodal microscopy with NanoJ-Fluidics
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Publication Type:Journal article
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Publication Sub Type:Article
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Authors:Almada P, Pereira PM, Culley S, Caillol G, Boroni-Rueda F, Dix CL, Charras G, Baum B, Laine RF, Leterrier C, Henriques R
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Publisher:Nature Publishing Group
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Publication date:15/03/2019
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Pagination:1223
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Journal:Nature Communications
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Volume:10
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Issue:1
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Status:Published online
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Country:England
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Print ISSN:2041-1723
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PII:10.1038/s41467-019-09231-9
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Language:eng
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Author URL:
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Full Text URL:
Abstract
Combining and multiplexing microscopy approaches is crucial to understand cellular events, but requires elaborate workflows. Here, we present a robust, open-source approach for treating, labelling and imaging live or fixed cells in automated sequences. NanoJ-Fluidics is based on low-cost Lego hardware controlled by ImageJ-based software, making high-content, multimodal imaging easy to implement on any microscope with high reproducibility. We demonstrate its capacity on event-driven, super-resolved live-to-fixed and multiplexed STORM/DNA-PAINT experiments.
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