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Publication Detail
Production and Concentration of Lentivirus for Transduction of Primary Human T Cells.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    Kennedy A, Cribbs AP
  • Publication date:
    2016
  • Pagination:
    85, 93
  • Journal:
    Methods Mol Biol
  • Volume:
    1448
  • Status:
    Published
  • Country:
    United States
  • Language:
    eng
  • Keywords:
    CD4+ T cells, CD45RA+, Human T cell, Lentiviral vector, Lentivirus, Primary, Titer, Gene Transfer Techniques, Genetic Vectors, HIV-1, Humans, Lentivirus, Primary Cell Culture, T-Lymphocytes, Transduction, Genetic, Transfection, Transgenes
Abstract
Lentiviral vectors have emerged as efficient tools for investigating T cell biology through their ability to efficiently deliver transgene expression into both dividing and nondividing cells. Such lentiviral vectors have the potential to infect a wide variety of cell types. However, despite this advantage, the ability to transduce primary human T cells remains challenging and methods to achieve efficient gene transfer are often time consuming and expensive. We describe a method for generating lentivirus that is simple to perform and does not require the purchase of non-standard equipment to transduce primary human T cells. Therefore, we provide an optimized protocol that is easy to implement and allow transduction with high efficiency and reproducibility.
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