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Publication Detail
Mixing Time in Intermediate-Sized Orbitally Shaken Reactors with Small Orbital Diameters
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Orbitally shaken reactors (OSR) are widely used in bioprocess development; however, a scaling law between large reactors, usually shaken in incubators at an orbital diameter of do = 1.5–5 cm, and microwell plates, shaken in benchtop thermomixers at do = 3 mm, is still missing. Here, the mixing time was measured in two reactors with the same volume but either cylindrical or square geometry for do = 3 mm. For such a small do, the acceleration mode to reach the final speed in the cylinder was found to greatly affect the free surface oscillations and thus the mixing time. The stepwise mode resulted in mixing times approximately six times smaller than in the direct mode. The natural frequency of the reactor, which is independent of do, was found to be an effective scaling parameter for systems with small do.
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