UCL  IRIS
Institutional Research Information Service
UCL Logo
Please report any queries concerning the funding data grouped in the sections named "Externally Awarded" or "Internally Disbursed" (shown on the profile page) to your Research Finance Administrator. Your can find your Research Finance Administrator at http://www.ucl.ac.uk/finance/research/post_award/post_award_contacts.php by entering your department
Please report any queries concerning the student data shown on the profile page to:

Email: portico-services@ucl.ac.uk

Help Desk: http://www.ucl.ac.uk/ras/portico/helpdesk
Publication Detail
Global gene expression responses of fission yeast to ionizing radiation.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    Watson A, Mata J, Bähler J, Carr A, Humphrey T
  • Publication date:
    02/2004
  • Pagination:
    851, 860
  • Journal:
    Mol Biol Cell
  • Volume:
    15
  • Issue:
    2
  • Status:
    Published
  • Country:
    United States
  • Print ISSN:
    1059-1524
  • PII:
    E03-08-0569
  • Language:
    eng
  • Keywords:
    Cell Cycle Proteins, Checkpoint Kinase 2, DNA Repair, DNA, Fungal, Furin, Gene Expression Regulation, Fungal, Mitogen-Activated Protein Kinases, Oligonucleotide Array Sequence Analysis, Protein Kinases, Radiation, Ionizing, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Signal Transduction
Abstract
A coordinated transcriptional response to DNA-damaging agents is required to maintain genome stability. We have examined the global gene expression responses of the fission yeast Schizosaccharomyces pombe to ionizing radiation (IR) by using DNA microarrays. We identified approximately 200 genes whose transcript levels were significantly altered at least twofold in response to 500 Gy of gamma IR in a temporally defined manner. The majority of induced genes were core environmental stress response genes, whereas the remaining genes define a transcriptional response to DNA damage in fission yeast. Surprisingly, few DNA repair and checkpoint genes were transcriptionally modulated in response to IR. We define a role for the stress-activated mitogen-activated protein kinase Sty1/Spc1 and the DNA damage checkpoint kinase Rad3 in regulating core environmental stress response genes and IR-specific response genes, both independently and in concert. These findings suggest a complex network of regulatory pathways coordinate gene expression responses to IR in eukaryotes.
Publication data is maintained in RPS. Visit https://rps.ucl.ac.uk
 More search options
UCL Researchers
Author
Genetics, Evolution & Environment
University College London - Gower Street - London - WC1E 6BT Tel:+44 (0)20 7679 2000

© UCL 1999–2011

Search by