UCL  IRIS
Institutional Research Information Service
UCL Logo
Please report any queries concerning the funding data grouped in the sections named "Externally Awarded" or "Internally Disbursed" (shown on the profile page) to your Research Finance Administrator. Your can find your Research Finance Administrator at http://www.ucl.ac.uk/finance/research/post_award/post_award_contacts.php by entering your department
Please report any queries concerning the student data shown on the profile page to:

Email: portico-services@ucl.ac.uk

Help Desk: http://www.ucl.ac.uk/ras/portico/helpdesk
Publication Detail
Global effects on gene expression in fission yeast by silencing and RNA interference machineries.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    Hansen KR, Burns G, Mata J, Volpe TA, Martienssen RA, Bähler J, Thon G
  • Publication date:
    01/2005
  • Pagination:
    590, 601
  • Journal:
    Mol Cell Biol
  • Volume:
    25
  • Issue:
    2
  • Status:
    Published
  • Country:
    United States
  • Print ISSN:
    0270-7306
  • PII:
    25/2/590
  • Language:
    eng
  • Keywords:
    Cell Cycle Proteins, Chromosomes, Fungal, Fungal Proteins, Gene Expression Profiling, Gene Expression Regulation, Fungal, Gene Silencing, Histone Deacetylases, Oligonucleotide Array Sequence Analysis, RNA Interference, Retroelements, Ribonuclease III, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Statistics as Topic, Terminal Repeat Sequences, Transcription, Genetic
Abstract
Histone modifications influence gene expression in complex ways. The RNA interference (RNAi) machinery can repress transcription by recruiting histone-modifying enzymes to chromatin, although it is not clear whether this is a general mechanism for gene silencing or whether it requires repeated sequences such as long terminal repeats (LTRs). We analyzed the global effects of the Clr3 and Clr6 histone deacetylases, the Clr4 methyltransferase, the zinc finger protein Clr1, and the RNAi proteins Dicer, RdRP, and Argonaute on the transcriptome of Schizosaccharomyces pombe (fission yeast). The clr mutants derepressed similar subsets of genes, many of which also became transcriptionally activated in cells that were exposed to environmental stresses such as nitrogen starvation. Many genes that were repressed by the Clr proteins clustered in extended regions close to the telomeres. Surprisingly few genes were repressed by both the silencing and RNAi machineries, with transcripts from centromeric repeats and Tf2 retrotransposons being notable exceptions. We found no correlation between repression by RNAi and proximity to LTRs, and the wtf family of repeated sequences seems to be repressed by histone deacetylation independent of RNAi. Our data indicate that the RNAi and Clr proteins show only a limited functional overlap and that the Clr proteins play more global roles in gene silencing.
Publication data is maintained in RPS. Visit https://rps.ucl.ac.uk
 More search options
UCL Researchers
Author
Genetics, Evolution & Environment
University College London - Gower Street - London - WC1E 6BT Tel:+44 (0)20 7679 2000

© UCL 1999–2011

Search by