Business services are customisable, interactive processes that providers have the potential to carry out together with clients that benefit from the effects. It is understood that business service transactions are best organised by means of virtual networks, where information technology allows for configuring multiple providers and processes on a per-request basis. Existing conceptual models for virtual service enterprises (VSEs) propose business service virtualisation for flexible and agile regulation and control of coordination between multiple providers and clients. In this paper, we present an approach for realising business service virtualisation based on software service technology. In particular, we propose a service-oriented software architecture for representing virtual business service processes as e-services. E-service models specify flexible business service interactions between multiple providers and clients of virtual service enterprises and allow for regulation and enforcement of their coordination. We demonstrate the utilisation of our e-service SOA in the context of an e-science scenario, where we show how to design e-service models for the use case of virtual polymorph prediction laboratories. © 2009 Springer-Verlag London Limited.