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Publication Detail
Cyclin-dependent kinase inhibits reinitiation of a normal S-phase program during G2 in fission yeast.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    Kiang L, Heichinger C, Watt S, Bähler J, Nurse P
  • Publication date:
  • Pagination:
    4025, 4032
  • Journal:
    Mol Cell Biol
  • Volume:
  • Issue:
  • Status:
  • Country:
    United States
  • PII:
  • Language:
  • Keywords:
    Cell Cycle Proteins, Cyclin B, Cyclin-Dependent Kinases, DNA Replication, Flow Cytometry, Fungal Proteins, G2 Phase, Gene Expression Regulation, Fungal, Genome, Fungal, Green Fluorescent Proteins, Microscopy, Fluorescence, Mutation, Oligonucleotide Array Sequence Analysis, S Phase, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, ras-GRF1
To achieve faithful replication of the genome once in each cell cycle, reinitiation of S phase is prevented in G(2) and origins are restricted from refiring within S phase. We have investigated the block to rereplication during G(2) in fission yeast. The DNA synthesis that occurs when G(2)/M cyclin-dependent kinase (CDK) activity is depleted has been assumed to be repeated rounds of S phase without mitosis, but this has not been demonstrated to be the case. We show here that on G(2)/M CDK depletion in G(2), repeated S phases are induced, which are correlated with normal G(1)/S transcription and attainment of doublings in cell size. Mostly normal mitotic S-phase origins are utilized, although at different efficiencies, and replication is essentially equal across the genome. We conclude that CDK inhibits reinitiation of S phase during G(2), and if G(2)/M CDK is depleted, replication results from induction of a largely normal S-phase program with only small differences in origin usage and efficiency.
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