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Publication Detail
Freeze-etching and x-ray diffraction of the isolated double-track layer from the cell wall of a gram-negative marine pseudomonad.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    Forge A, Costerton JW, Kerr KA
  • Publication date:
    01/1973
  • Pagination:
    445, 451
  • Journal:
    J Bacteriol
  • Volume:
    113
  • Issue:
    1
  • Status:
    Published
  • Country:
    United States
  • Print ISSN:
    0021-9193
  • Language:
    eng
  • Keywords:
    Bacteria, Cell Wall, Freeze Etching, Marine Biology, Microscopy, Electron, Pseudomonas, Seawater, Staining and Labeling, Water Microbiology, X-Ray Diffraction
Abstract
The isolated double-track layer of the cell wall of the gram-negative marine pseudomonad studied here contains a cleavage plane. This finding localizes the single cleavage plane of the cell wall and shows that the molecular architecture of this layer provides the lipid-enriched layer which cleaves preferentially in the frozen cell. The observation that the isolated double-track layer of the cell wall is sufficiently ordered at the molecular level to yield a well-defined X-ray diffraction pattern with a d-spacing of 0.44 nm shows that its molecular architecture is very similar to that of true membranes. This specific d-spacing is produced by the highly ordered packing of the hydrophobic portions of phospholipid molecules. Therefore, the double-track layer of the cell wall has been shown, by these two biophysical means, to have a molecular architecture which would allow it to function as the membrane-like "molecular sieve" layer, whose presence has been deduced from physiological data. This layer is important in the retention of cell wall-associated enzymes and in the control of the movement of large molecules through the cell wall.
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