UCL  IRIS
Institutional Research Information Service
UCL Logo
Please report any queries concerning the funding data grouped in the sections named "Externally Awarded" or "Internally Disbursed" (shown on the profile page) to your Research Finance Administrator. Your can find your Research Finance Administrator at https://www.ucl.ac.uk/finance/research/rs-contacts.php by entering your department
Please report any queries concerning the student data shown on the profile page to:

Email: portico-services@ucl.ac.uk

Help Desk: http://www.ucl.ac.uk/ras/portico/helpdesk
Publication Detail
Membrane stains as an objective means to distinguish isolated inner and outer hair cells.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    Zajic G, Forge A, Schacht J
  • Publication date:
    03/1993
  • Pagination:
    53, 57
  • Journal:
    Hear Res
  • Volume:
    66
  • Issue:
    1
  • Status:
    Published
  • Country:
    Netherlands
  • Print ISSN:
    0378-5955
  • PII:
    0378-5955(93)90259-4
  • Language:
    eng
  • Keywords:
    Animals, Carbocyanines, Fluorescent Dyes, Guinea Pigs, Hair Cells, Auditory, Hair Cells, Auditory, Inner, Microscopy, Fluorescence, Rhodamines, Staining and Labeling
Abstract
The use of isolated cochlear outer and inner hair cells has become widespread. While the morphological features of these two cell types in general are sufficiently different to allow discrimination, there are situations where confusion can arise. Small outer hair cells, particularly when they are swollen or distorted, can take on an appearance suggestive of inner hair cells. We describe here two fluorescent membrane stains, 3,3'-dihexyloxacarbocyanine iodide and rhodamine B hexyl ester, as an objective means to distinguish between cochlear hair cell types. Both stains mark the subsurface cisternae of outer hair cells thereby delineating the cell outline, and the interior of the cell shows discrete structure. On the other hand, in inner hair cells, the outline of the cell is not resolved while the interior is diffusely fluorescent. Since the two probes have different excitation and emission wavelengths (fluorescein- and rhodamine-like, respectively), this staining procedure can even be used in the presence of another fluorescent marker (for example, a calcium-indicating dye) by appropriate choice of the membrane stain.
Publication data is maintained in RPS. Visit https://rps.ucl.ac.uk
 More search options
UCL Researchers
Author
The Ear Institute
University College London - Gower Street - London - WC1E 6BT Tel:+44 (0)20 7679 2000

© UCL 1999–2011

Search by