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Publication Detail
Direct degradation of articular cartilage by rheumatoid synovial fluid: contribution of proteolytic enzymes.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    Larbre JP, Moore AR, Da Silva JA, Iwamura H, Ioannou Y, Willoughby DA
  • Publication date:
  • Pagination:
    1796, 1801
  • Journal:
    J Rheumatol
  • Volume:
  • Issue:
  • Status:
  • Country:
  • Print ISSN:
  • Language:
  • Keywords:
    Adult, Aged, Arthritis, Rheumatoid, Cartilage, Articular, Female, Glycosaminoglycans, Humans, Iodoacetamide, Male, Middle Aged, Osteoarthritis, Pancreatic Elastase, Pepstatins, Peptide Hydrolases, Synovial Fluid
OBJECTIVE: To test the effects of synovial fluids (SF) on human cartilage in an in vitro model. METHODS: Freshly collected SF were incubated with cryostat sections of articular cartilage, and glycosaminoglycan (GAG) loss determined by microdensitometry after alcian blue staining. RESULTS: Of 20 rheumatoid SF, 11 induced significant GAG loss compared with only 3 out of 15 osteoarthritic SF. The effect of rheumatoid fluids appeared to be related to disease activity. GAG loss was partially prevented by a broad spectrum serine protease inhibitor and a specific elastase inhibitor. Cartilage degrading activity was lost on storage which may explain why it has not been widely reported before. CONCLUSION: Rheumatoid SF can directly degrade cartilage through the action of proteases. There is an involvement of serine proteases, elastase in particular.
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