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Publication Detail
vLUME: 3D Virtual Reality for Single-molecule Localization Microscopy
  • Publication Type:
    Journal article
  • Publication Sub Type:
  • Authors:
    Spark A, Kitching A, Esteban-Ferrer D, Handa A, Carr A, Needham L-M, Ponjavic A, Santos MDC, McColl J, Leterrier C, Davis S, Henriques R, Lee S
  • Publication date:
  • Status:
Super-Resolution (SR) Microscopy based on 3D Single-Molecule Localization Microscopy (SMLM) is now well established 1,2 and its wide-spread adoption has led to the development of more than 36 software packages, dedicated to quantitative evaluation of the spatial and temporal detection of fluorophore photoswitching 3 . While the initial emphasis in the 3D SMLM field has clearly been on improving resolution and data quality, there is now a marked absence of 3D visualization approaches that enable the straightforward, high-fidelity exploration of this type of data. Inspired by the horological phosphorescence points that illuminate watch-faces in the dark, we present v LUME (Visualization of the Universe in a Micro Environment, pronounced ‘volume’) a free-for-academic-use immersive virtual reality-based (VR) visualization software package purposefully designed to render large 3D-SMLM data sets. v LUME enables robust visualization, segmentation and quantification of millions of fluorescence puncta from any 3D SMLM technique. v LUME has an intuitive user-interface and is compatible with all commercial VR hardware (Oculus Rift/Quest and HTC Vive, Supplementary Video 1). v LUME accelerates the analysis of highly complex 3D point-cloud data and the rapid identification of defects that are otherwise neglected in global quality metrics.
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