UCL  IRIS
Institutional Research Information Service
UCL Logo
Please report any queries concerning the funding data grouped in the sections named "Externally Awarded" or "Internally Disbursed" (shown on the profile page) to your Research Finance Administrator. Your can find your Research Finance Administrator at https://www.ucl.ac.uk/finance/research/rs-contacts.php by entering your department
Please report any queries concerning the student data shown on the profile page to:

Email: portico-services@ucl.ac.uk

Help Desk: http://www.ucl.ac.uk/ras/portico/helpdesk
Publication Detail
Astigmatic traction force microscopy (aTFM).
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Article
  • Authors:
    Li D, Colin-York H, Barbieri L, Javanmardi Y, Guo Y, Korobchevskaya K, Moeendarbary E, Li D, Fritzsche M
  • Publication date:
    12/04/2021
  • Pagination:
    2168
  • Journal:
    Nat Commun
  • Volume:
    12
  • Issue:
    1
  • Status:
    Published
  • Country:
    England
  • PII:
    10.1038/s41467-021-22376-w
  • Language:
    eng
Abstract
Quantifying small, rapidly progressing three-dimensional forces generated by cells remains a major challenge towards a more complete understanding of mechanobiology. Traction force microscopy is one of the most broadly applied force probing technologies but ascertaining three-dimensional information typically necessitates slow, multi-frame z-stack acquisition with limited sensitivity. Here, by performing traction force microscopy using fast single-frame astigmatic imaging coupled with total internal reflection fluorescence microscopy we improve the temporal resolution of three-dimensional mechanical force quantification up to 10-fold compared to its related super-resolution modalities. 2.5D astigmatic traction force microscopy (aTFM) thus enables live-cell force measurements approaching physiological sensitivity.
Publication data is maintained in RPS. Visit https://rps.ucl.ac.uk
 More search options
UCL Researchers
Author
Dept of Mechanical Engineering
Author
Dept of Mechanical Engineering
University College London - Gower Street - London - WC1E 6BT Tel:+44 (0)20 7679 2000

© UCL 1999–2011

Search by