UCL  IRIS
Institutional Research Information Service
UCL Logo
Please report any queries concerning the funding data grouped in the sections named "Externally Awarded" or "Internally Disbursed" (shown on the profile page) to your Research Finance Administrator. Your can find your Research Finance Administrator at https://www.ucl.ac.uk/finance/research/rs-contacts.php by entering your department
Please report any queries concerning the student data shown on the profile page to:

Email: portico-services@ucl.ac.uk

Help Desk: http://www.ucl.ac.uk/ras/portico/helpdesk
Publication Detail
B-cell class switching in trans- and cis-gendered healthy young people is differentially influenced by sex hormones
  • Publication Type:
    Conference presentation
  • Publication Sub Type:
    Presentation
  • Authors:
    Peckham H, Rosser EC, Radziszewska A, Robinson G, Martin L, De Gruijter N, Jury E, Butler G, Ciurtin C
  • Name of Conference:
    48th Meeting of the British Society for Paediatric Endocrinology and Diabetes
  • Conference place:
    Virtual
  • Conference start date:
    24/11/2021
  • Conference finish date:
    26/11/2021
  • Keywords:
    Hormones, Endocrinology, Immunology
  • Addresses:
    Hannah Peckham
    Centre for Adolescent Rheumatology Versus Arthritis at UCL, UCLH and GOSH
    Division of Medicine
    5 University Street
    London
    WC1E 6JF
    United Kingdom
Abstract
Cis-gender females are known to mount stronger humoral immune responses than cis-gender males. Little is known about the immunophenotypes of transgender individuals on gender-affirming hormonal treatment, despite growing evidence that hormones influence the immune system. Via the process of class-switch recombination (CSR), B-cell immunoglobulin isotype ‘switches’ from early IgM/IgD classes to IgG/IgA/IgE. Whilst important in infection/vaccine responses; switched isotypes play a significant role in autoimmunity. T-follicular helper cells (Tfh) aid B-cells in CSR. This study investigates the impact of sex-chromosomal complement and hormonal milieu on B-cell CSR, using samples from healthy cis- and trans-gendered individuals to create an in vivo, age-adjusted model of these effects. Peripheral blood samples were collected with informed consent from cis-gender male (n=35) and female (n=53) post-pubertal volunteers (14-28y), and trans-gender male (on testosterone and/or GnRH-analogue; n=25) and female (on oestradiol and/or GnRHa; n=19) volunteers (16-19y). In-depth phenotyping of peripheral blood mononuclear cells was performed using multiparameter flow cytometry. GraphPad Prism was used for statistical testing appropriate to the data distribution/number of groups (unpaired t-test/Mann-Whitney U, one-way ANOVA/ Kruskal-Wallis). Cis-females had greater percentages of class-switched B-cells than cis-males. In trans-males, GnRHa monotreatment was sufficient to also significantly decrease the levels of class-switched B-cells, and additional testosterone treatment saw no further decrease. GnRHa treatment, with or without oestradiol in trans-females, however, was not associated with the increase seen in cis-females. Indeed, trans-females had the lowest percentages of class-switched B-cells of all groups. These patterns were replicated when B-cells were stained specifically for IgG/A/M/D. Cis-females and -males had similar levels of Tfh cells. Both trans-females and trans-males however, trended toward lower levels of Tfh cells, with little distinction between GnRHa-only and gender-affirming hormones. Sex hormones may differentially affect humoral immune responses of those assigned female at birth versus those assigned male. Whilst trans-males followed the same decreased class-switching pattern as cis-males, a surprising and significant decrease was seen in trans-females, that did not mirror the immune phenotype of cis-females. These data support the need for further research into the interactions between hormones and chromosomal sex, as immunological outcomes in trans-gender people may differ from those in cis-gendered individuals.
Publication data is maintained in RPS. Visit https://rps.ucl.ac.uk
 More search options
UCL Researchers Show More
Author
Div of Medicine
Author
Inflammation
Author
Div of Medicine
Author
Div of Medicine
Author
Inflammation
University College London - Gower Street - London - WC1E 6BT Tel:+44 (0)20 7679 2000

© UCL 1999–2011

Search by