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Publication Detail
Structural investigations of native and oxidised LDL using X-ray and neutron solution scattering techniques
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    Meyer DF, Nealis AS, Bruckdorfer KR, Perkins SJ
  • Publication date:
  • Pagination:
    257, 260
  • Journal:
    Journal De Physique. IV : JP
  • Volume:
  • Issue:
  • Status:
  • Print ISSN:
The gross structures of both the native and oxidised (atherogenic) forms of low density lipoproteins (LDL) were compared using neutron and X-ray solution scattering. Neutron data in 2H2O solutions showed that native LDL is well represented as a quasispherical structure, while X-ray data showed that the internal structure of LDL has lipid in the core and apo-B100 protein and phospholipid headgroups on the surface. When LDL oxidation was induced using a ratio of 6.4 μM Cu2+ to 1 mg/ml LDL protein, structural disintegration of LDL was observed in the scattering curves over a 72 hour period. This disintegration was parallelled by an increase in the RG and I(0)/c parameters observed by neutron and X-ray Guinier analyses, and changes in the concentric shell-like structure of the lipids in the core as observed in the distance distribution functions. Structural changes included the aggregation and degradation of LDL, as confirmed by subsequent SDS-polyacrylamide gel electrophoresis. These changes occurred after the antioxidant content of LDL had been fully depleted.
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