UCL  IRIS
Institutional Research Information Service
UCL Logo
Please report any queries concerning the funding data grouped in the sections named "Externally Awarded" or "Internally Disbursed" (shown on the profile page) to your Research Finance Administrator. Your can find your Research Finance Administrator at https://www.ucl.ac.uk/finance/research/rs-contacts.php by entering your department
Please report any queries concerning the student data shown on the profile page to:

Email: portico-services@ucl.ac.uk

Help Desk: http://www.ucl.ac.uk/ras/portico/helpdesk
Publication Detail
A human anti-dsDNA monoclonal antibody caused hyaline thrombi formation in kidneys of 'leaky' SCID mice
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Article
  • Authors:
    Mason LJ, Ravirajan CT, Latchman DS, Isenberg DA
  • Publication date:
    10/2001
  • Pagination:
    137, 142
  • Journal:
    Clinical and Experimental Dermatology
  • Volume:
    126
  • Issue:
    1
  • Print ISSN:
    0307-6938
  • Keywords:
    antibodies, Antibody, formation, Kidney, kidneys, MB, mice, MONOCLONAL ANTIBODIES, MONOCLONAL ANTIBODY, MONOCLONAL-ANTIBODIES, MONOCLONAL-ANTIBODY, SCID MICE, abnormal, age, Animal, anti-DNA, anti-DNA antibodies, antibodies, Antibodies, Antinuclear, Monoclonal, ANTINUCLEAR, antiphospholipid, As, assessment, B CELL, B CELLS, B-cell, B-CELLS, Capillaries, cell, CELLS, CLONE, CLONES, control, cytology, Damage, DEPOSITION, DNA, Electron, electron microscopy, etiology, FIBRIN, functional, Hyalin, Hybridomas, IgG, IM, immune, Immune System, IMMUNE-SYSTEM, IMMUNOGLOBULIN, IMMUNOGLOBULINS, immunology, in vivo, in-vivo, LA, LIGHT, light microscopy, Lupus Erythematosus, Systemic, LYMPHOCYTE, MATURE, Mice, Inbred BALB C, Scid, Microscopy, OLDER, pathogenicity, pathology, pharmacology, Proteinuria, Rejection, Result, Rheumatology, Support, Non-U.S.Gov't, SYSTEM, Thrombosis, TIME, Use, vivo
  • Notes:
    UI - 21536507 LA - eng RN - 0 (Antibodies, Antinuclear) RN - 0 (Antibodies, Monoclonal) RN - 9007-49-2 (DNA) PT - Journal Article DA - 20011026 IS - 0009-9104 SB - IM CY - England
Abstract
There are few studies assessing the pathogenicity of human monoclonal anti-DNA antibodies. The use of SCID mice avoids the problem of rejection of the human hybridoma cells thus allowing in vivo assessment of human immunoglobulins. Using electron microscopy we have shown that the human IgG anti-dsDNA monoclonal antibody, RH14, is nephritogenic in SCID mice, causing morphological changes in the kidney due to immunoglobulin deposition. The problem with using SCID mice is that they have an abnormal immune system; normally they are used at about 2 months of age, at which time they have virtually no functional T or B cells. It is known that older SCID mice become increasingly 'leaky', that is they develop some mature lymphocyte clones. Our aim was to assess if implanting anti-DNA antibodies into older 'leaky' SCID mice would result in pathology which was observable by light microscopy. Eight-month-old SCID mice were implanted with human hybridoma cells secreting either RH14 an anti-dsDNA IgG, CL24, an antiphospholipid antibody or an irrelevant human IgG control. As previously, RH14 deposited in the kidney and caused proteinuria but unexpectedly we also observed hyaline thrombi in the kidney glomeruli and peritubular capillaries. These thrombi occurred only in the case of RH14 implanted mice and were found to stain positively for human IgG and fibrin. However, apart from the interesting thrombi, we did not observe any greater pathological damage resulting from the anti-dsDNA antibody deposition than we had seen in the younger mice; indeed, the electron microscopic findings were more limited
Publication data is maintained in RPS. Visit https://rps.ucl.ac.uk
 More search options
UCL Researchers
Author
ICH - Directors Office
University College London - Gower Street - London - WC1E 6BT Tel:+44 (0)20 7679 2000

© UCL 1999–2011

Search by