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Publication Detail
Generation and application of TGFβ-educated human Vγ9Vδ2 T cells
  • Publication Type:
    Journal article
  • Authors:
    Parente-Pereira AC, Beatson RE, Davies DM, Hull C, Whilding LM, Porter JC, Maher J
  • Publisher:
    Elsevier BV
  • Publication date:
    17/06/2022
  • Journal:
    STAR Protocols
  • Volume:
    3
  • Issue:
    2
  • Article number:
    101319
  • Status:
    Published
  • Print ISSN:
    2666-1667
  • Language:
    English
  • Notes:
    ©2022 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Abstract
Clinical trials that tested the antitumor activity of γδ T cells have been mostly unsuccessful. To address this, we expanded human Vγ9Vδ2 T cells in TGFβ1, a cytokine which enhances their viability, trafficking properties, and intrinsic antitumor activity. This protocol summarizes the production and in vitro functional characterization of TGFβ1 educated human Vγ9Vδ2 cells and highlights their compatibility with chimeric antigen receptor (CAR) engineering. We also describe in vivo testing of the antitumor activity of these CAR T cells in mice. For complete details on the use and execution of this protocol, please refer to Beatson et al. (2021).
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