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Publication Detail
Membrane Capacitance Measurements of Stimulus-Evoked Exocytosis in Adrenal Chromaffin Cells.
  • Publication Type:
    Journal article
  • Publication Sub Type:
  • Authors:
    Seward EP, Wykes RC
  • Publisher:
    Humana Press
  • Publication date:
  • Pagination:
    187, 202
  • Journal:
    Methods in Molecular Biology
  • Volume:
  • Status:
  • Country:
    United States
  • Print ISSN:
  • Language:
  • Keywords:
    Calcium channels, Capacitance measurements, Chromaffin cells, Endocytosis, Exocytosis, IRP, RRP, SRP, Calcium, Calcium Channels, Cells, Cultured, Chromaffin Cells, Exocytosis, Patch-Clamp Techniques
Research using membrane capacitance (Cm) measurements in adrenal chromaffin cells has transformed our understanding of the molecular mechanisms controlling regulated exocytosis. This is in part due to the exquisite temporal resolution of the technique, and the possibility of combining quantification of exo-/endocytosis at the whole-cell level, with the ability to simultaneously monitor and control the calcium signals triggering vesicle fusion. In this regard, experiments performed with Cm measurements complement amperometry experiments that give a measure of secreted transmitter and the behavior of the fusion pore, and fluorescent microscopy studies used to monitor vesicle and protein dynamics in imaged regions of the cell. In this chapter, we provide a detailed account of the methodology used to perform whole-cell patch clamp measurements of Cm in combination with voltage-clamp recordings of voltage-gated calcium channels to quantify stimulus-secretion coupling in chromaffin cells. Stimulus protocols developed for investigation of functionally distinct releasable vesicle pools are also described.
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