Institutional Research Information Service
UCL Logo
Please report any queries concerning the funding data grouped in the sections named "Externally Awarded" or "Internally Disbursed" (shown on the profile page) to your Research Finance Administrator. Your can find your Research Finance Administrator at https://www.ucl.ac.uk/finance/research/rs-contacts.php by entering your department
Please report any queries concerning the student data shown on the profile page to:

Email: portico-services@ucl.ac.uk

Help Desk: http://www.ucl.ac.uk/ras/portico/helpdesk
Publication Detail
Single cell mechanotransduction and its modulation analyzed by atomic force microscope indentation
  • Publication Type:
    Journal article
  • Publication Sub Type:
  • Authors:
    Charras GT, Horton MA
  • Publication date:
  • Pagination:
    2970, 2981
  • Journal:
    Biophysical Journal
  • Volume:
  • Issue:
  • Print ISSN:
  • Keywords:
    ACID, ACTIVATION, adaptation, Adaptation, Physiological, analysis, Animal, As, Biomechanics, Biophysics, bone, calcium, Calcium Signaling, cell, CELL RESPONSES, CELLS, Cells, Cultured, Cellular, channel, channels, COMPONENT, COMPONENTS, Confocal, connexin, connexins, cytology, Cytoskeleton, detection, Disruption, distribution, drug effects, Egtazic Acid, Force, gap junctions, IM, INTACT, ion, ion channel, Ion Channels, LA, LEVEL, link, May, mechanical, MECHANICAL STRAIN, mechanotransduction, membrane, Microscopy, Atomic Force, Microscopy, Confocal, model, Models, Biological, modulation, nature, network, osteoblasts, Other, PACLITAXEL, PATHWAY, PATHWAYS, pharmacology, physiology, rats, RELAXATION, response, RESPONSES, Result, SINGLE, Strain, STRAINS, stress, Support, Non-U.S.Gov't, technique, transduction, transmission, treatment, Treatments, Vimentin
  • Notes:
    UI - 22017748 DA - 20020522 IS - 0006-3495 LA - eng PT - Journal Article RN - 0 (Connexins) RN - 33069-62-4 (Paclitaxel) RN - 67-42-5 (Egtazic Acid) SB - IM
The skeleton adapts to its mechanical usage, although at the cellular level, the distribution and magnitude of strains generated and their detection are ill-understood. The magnitude and nature of the strains to which cells respond were investigated using an atomic force microscope (AFM) as a microindentor. A confocal microscope linked to the setup enabled analysis of cellular responses. Two different cell response pathways were identified: one, consequent upon contact, depended on activation of stretch-activated ion channels; the second, following stress relaxation, required an intact microtubular cytoskeleton. The cellular responses could be modulated by selectively disrupting cytoskeletal components thought to be involved in the transduction of mechanical stimuli. The F-actin cytoskeleton was not required for responses to mechanical strain, whereas the microtubular and vimentin networks were. Treatments that reduced membrane tension, or its transmission, selectively reduced contact reactions. Immunostaining of the cell cytoskeleton was used to interpret the results of the cytoskeletal disruption studies. We provide an estimate of the cellular strain magnitude needed to elicit intracellular calcium responses and propose a model that links single cell responses to whole bone adaptation. This technique may help to understand adaptation to mechanical usage in other organs
Publication data is maintained in RPS. Visit https://rps.ucl.ac.uk
 More search options
UCL Researchers
London Centre for Nanotechnology
University College London - Gower Street - London - WC1E 6BT Tel:+44 (0)20 7679 2000

© UCL 1999–2011

Search by