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Publication Detail
Tomographic imaging of flourescence resonance energy transfer in highly light scattering media
  • Publication Type:
    Conference
  • Authors:
    Soloviev VY, McGinty J, Tahir KB, Laine R, Stuckey DW, Mohan PS, Hajnal JV, Sardini A, French PMW, Arridge SR
  • Publisher:
    SPIE - The International Society for Optical Engineering
  • Publication date:
    2010
  • Place of publication:
    Bellingham, US
  • Published proceedings:
    Proceedings of SPIE
  • Volume:
    7573
  • Status:
    Published
  • Notes:
    Article 75730G. Conference Title: Biomedical Applications of Light Scattering IV
Abstract
Three-dimensional localization of protein conformation changes in turbid media using Frster Resonance Energy Transfer (FRET) was investigated by tomographic fluorescence lifetime imaging (FLIM). FRET occurs when a donor fluorophore, initially in its electronic excited state, transfers energy to an acceptor fluorophore in close proximity through non-radiative dipole-dipole coupling. An acceptor effectively behaves as a quencher of the donor's fluorescence. The quenching process is accompanied by a reduction in the quantum yield and lifetime of the donor fluorophore. Therefore, FRET can be localized by imaging changes in the quantum yield and the fluorescence lifetime of the donor fluorophore. Extending FRET to diffuse optical tomography has potentially important applications such as in vivo studies in small animal. We show that FRET can be localized by reconstructing the quantum yield and lifetime distribution from time-resolved non-invasive boundary measurements of fluorescence and transmitted excitation radiation. Image reconstruction was obtained by an inverse scattering algorithm. Thus we report, to the best of our knowledge, the first tomographic FLIM-FRET imaging in turbid media. The approach is demonstrated by imaging a highly scattering cylindrical phantom concealing two thin wells containing cytosol preparations of HEK293 cells expressing TN-L15, a cytosolic genetically-encoded calcium FRET sensor. A 10mM calcium chloride solution was added to one of the wells to induce a protein conformation change upon binding to TN-L15, resulting in FRET and a corresponding decrease in the donor fluorescence lifetime. The resulting fluorescence lifetime distribution, the quantum efficiency, absorption and scattering coefficients were reconstructed.
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