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Publication Detail
The receptor subunits generating NMDA receptor mediated currents in oligodendrocytes.
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Journal Article
  • Authors:
    Burzomato V, Frugier G, Pérez-Otaño I, Kittler JT, Attwell D
  • Publication date:
    15/09/2010
  • Pagination:
    3403, 3414
  • Journal:
    J Physiol
  • Volume:
    588
  • Issue:
    Pt 18
  • Status:
    Published
  • Country:
    England
  • PII:
    jphysiol.2010.195503
  • Language:
    eng
  • Keywords:
    Animals, Cerebellum, Electrophysiological Phenomena, Evoked Potentials, Gene Expression Regulation, HEK293 Cells, Humans, Magnesium, Oligodendroglia, Protein Isoforms, Protein Subunits, Rats, Receptors, N-Methyl-D-Aspartate
Abstract
NMDA receptors have been shown to contribute to glutamate-evoked currents in oligodendrocytes. Activation of these receptors damages myelin in ischaemia, in part because they are more weakly blocked by Mg(2+) than are most neuronal NMDA receptors. This weak Mg(2+) block was suggested to reflect an unusual subunit composition including the NR2C and NR3A subunits. Here we expressed NR1/NR2C and triplet NR1/NR2C/NR3A recombinant receptors in HEK cells and compared their currents with those of NMDA-evoked currents in rat cerebellar oligodendrocytes. NR1/NR2C/3A receptors were less blocked by 2 mM Mg(2+) than were NR1/NR2C receptors (the remaining current was 30% and 18%, respectively, of that seen without added Mg(2+)) and showed less channel noise, suggesting a smaller single channel conductance. NMDA-evoked currents in oligodendrocytes showed a Mg(2+) block (to 32%) similar to that observed for NR1/NR2C/NR3A and significantly different from that for NR1/NR2C receptors. Co-immunoprecipitation revealed interactions between NR1, NR2C and NR3A subunits in a purified myelin preparation from rat brain. These data are consistent with NMDA-evoked currents in oligodendrocytes reflecting the activation of receptors containing NR1, NR2C and NR3A subunits.
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