Institutional Research Information Service
UCL Logo
Please report any queries concerning the funding data grouped in the sections named "Externally Awarded" or "Internally Disbursed" (shown on the profile page) to your Research Finance Administrator. Your can find your Research Finance Administrator at https://www.ucl.ac.uk/finance/research/rs-contacts.php by entering your department
Please report any queries concerning the student data shown on the profile page to:

Email: portico-services@ucl.ac.uk

Help Desk: http://www.ucl.ac.uk/ras/portico/helpdesk
Publication Detail
Molecular expression systems for anti-DNA antibodies--2
  • Publication Type:
    Journal article
  • Publication Sub Type:
  • Authors:
    Kumar S, Kalsi JK, Ravirajan CT, Latchman DS, Pearl LH, Isenberg DA
  • Publication date:
  • Pagination:
    833, 842
  • Journal:
  • Volume:
  • Issue:
  • Print ISSN:
  • Keywords:
    ABILITY, ACID, activity, anti-DNA, antibodies, Antibody, Antibody Fragments, ANTIGEN, Antigens, As, attributes, Autoantibodies, BINDING, cell, COMPLEX, COMPLEXES, Crystallization, development, DIFFICULT, DNA, DNA-binding, DNA-BINDING ACTIVITY, DRUG, DRUGS, experimental, expression, FOCUS, FRAGMENT, FRAGMENTS, functional, IM, INHIBIT, INSIGHT, interaction, LA, Lupus, MARKER, Markers, Molecular, novel, OVEREXPRESSION, PATHOGENESIS, pathogenicity, PROTEIN, PURIFICATION, QUANTITY, relationship, renal, Review, SITE, SPECIFICITIES, SPECIFICITY, structural, Structure, SYSTEM, SYSTEMIC, SYSTEMIC LUPUS ERYTHEMATOSUS, SYSTEMIC-LUPUS-ERYTHEMATOSUS, SYSTEMS, THERAPIES, therapy, Three-dimensional, understanding
  • Notes:
    UI - 22416389 DA - 20030116 IS - 0961-2033 LA - eng PT - Journal Article SB - IM
Antibodies to double-stranded DNA are the best-known serological markers of systemic lupus erythematosus, and are closely associated with its renal pathogenesis. How these antibodies recognize DNA is not fully understood. An understanding of the relationship between the functional attributes of an antibody with the three-dimensional structure of its antigen-combining site would allow an insight into the rules that dictate auto-antibody-nucleic acid interaction and consequent pathogenicity of the autoantibody. Data from such studies could assist the development of novel drugs as an approach to specific therapies that can inhibit or disrupt protein-nucleic acid interactions. A full understanding of the binding specificities can be achieved only by experimental determination of detailed three-dimensional structure of these antibodies alone, and of their complexes with specific DNA antigens. A prerequisite of such a study is the ability to produce multimilligram quantities of the antibody protein. However, these antibodies are particularly difficult to express, probably due to their DNA-binding activity. This review attempts to focus on the recent developments on the over-expression of anti-DNA antibody fragments in heterologous cell expression systems and their purification to homogeneity that would in turn allow their structural studies via crystallization
Publication data is maintained in RPS. Visit https://rps.ucl.ac.uk
 More search options
UCL Researchers
ICH - Directors Office
University College London - Gower Street - London - WC1E 6BT Tel:+44 (0)20 7679 2000

© UCL 1999–2011

Search by