UCL  IRIS
Institutional Research Information Service
UCL Logo
Please report any queries concerning the funding data grouped in the sections named "Externally Awarded" or "Internally Disbursed" (shown on the profile page) to your Research Finance Administrator. Your can find your Research Finance Administrator at https://www.ucl.ac.uk/finance/research/rs-contacts.php by entering your department
Please report any queries concerning the student data shown on the profile page to:

Email: portico-services@ucl.ac.uk

Help Desk: http://www.ucl.ac.uk/ras/portico/helpdesk
Publication Detail
Molecular expression systems for anti-DNA antibodies--1
  • Publication Type:
    Journal article
  • Publication Sub Type:
    Article
  • Authors:
    Rahman A, Haley J, Latchman DS
  • Publication date:
    2002
  • Pagination:
    824, 832
  • Journal:
    Lupus
  • Volume:
    11
  • Issue:
    12
  • Print ISSN:
    0961-2033
  • Keywords:
    anti-DNA, antibodies, Antibody, Antibody Fragments, Arginine, ASSAY, BINDING, CHAIN, cloning, DNA, experiments, expression, FRAGMENT, FRAGMENTS, function, IM, LA, LIGHT, LIGHT-CHAIN, Methods, Molecular, murine, Mutagenesis, PAPER, pathogenicity, play, product, PRODUCTS, Properties, Property, QUANTITY, RANGE, REPERTOIRE, Review, REVIEWS, SEQUENCE, SEQUENCES, Structure, SYSTEM, SYSTEMS
  • Notes:
    UI - 22416388 LA - eng PT - Journal Article DA - 20030116 IS - 0961-2033 SB - IM CY - England
Abstract
Molecular expression systems can be used to produce whole antibodies or antibody fragments. The properties of these expression products can be tested in assays of binding or pathogenicity. Expression systems can be used to produce large quantities of antibodies which are already well- characterized, to produce new antibodies by repertoire cloning, or to produce slight modifications in the sequences of antibodies by mutagenesis prior to expression. This paper reviews the ways in which these methods have been used to study the structure and function of human and murine anti-DNA antibodies. A consistent finding, from experiments using a range of different expression methods and antibodies, is that sequence motifs including arginine residues play a major role in binding to DNA. These motifs can be present on either the heavy or the light chain, but are particularly reported in V(H)CDR3
Publication data is maintained in RPS. Visit https://rps.ucl.ac.uk
 More search options
UCL Researchers
Author
ICH - Directors Office
University College London - Gower Street - London - WC1E 6BT Tel:+44 (0)20 7679 2000

© UCL 1999–2011

Search by